HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper)

The HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper) from Vazyme is designed for first-strand cDNA synthesis from total RNA or poly(A)+ RNA templates. It is suitable for downstream applications such as RT-PCR, RT-qPCR, gene expression analysis and long-fragment cDNA amplification.

Product overview

This fourth-generation HiScript kit provides improved reverse transcription efficiency, stronger extension ability, faster reaction speed and higher tolerance to inhibitors compared with previous versions. The kit contains a genomic DNA elimination module, allowing contaminating genomic DNA to be removed before reverse transcription for more reliable downstream analysis.

Key features

• First-strand cDNA synthesis kit for total RNA or poly(A)+ RNA templates
• Fourth-generation HiScript reverse transcription chemistry
• Includes gDNA wiper module for genomic DNA removal
• Fast genomic DNA elimination at 42°C for 2 minutes
• Efficient reverse transcription in as little as 5 minutes
• Enhanced extension capability for long cDNA synthesis
• Supports cDNA synthesis up to 15 kb
• Higher reverse transcription efficiency for lower Ct values in downstream qPCR
• Improved tolerance to inhibitors and challenging RNA samples
• Suitable for downstream qPCR quantification and long-fragment cDNA amplification

Applications

• First-strand cDNA synthesis
• Reverse transcription
• RT-PCR
• RT-qPCR
• Gene expression analysis
• Long-fragment cDNA amplification
• RNA template conversion for downstream PCR workflows

Input material

• Total RNA
• Poly(A)+ RNA