Human CRP ELISA Kit

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Cytodiagnostics

SKU:EL-009

Regular price €465,00 EUR
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The Human CRP ELISA Kit is a quantitative solid-phase sandwich ELISA for measuring human C-reactive protein, also known as CRP or pentraxin 1, in research samples. The kit is designed for use with human serum, plasma and cell culture supernatant samples.

Key features

  • Quantitative sandwich ELISA for human CRP / C-reactive protein
  • Suitable sample types: serum, plasma and cell culture supernatant
  • Pre-coated 96-well plate with breakable 12 x 8 well strips
  • Ready-to-use workflow: add standards and samples directly to wells
  • No lengthy blocking or rehydration steps required
  • Colorimetric detection using a microplate reader
  • Sample volume: 100 µl
  • Assay length: approximately 4.5 hours
  • Assay range: 31.2–2000 pg/ml
  • Analytical sensitivity: <10 pg/ml
  • Intra-assay CV: <5%
  • Inter-assay CV: <6.5%

Target information

C-reactive protein is an acute-phase inflammatory protein of the pentraxin family. It is widely studied in inflammation, immune response, infection, cardiovascular research and other biomedical research contexts. This kit is intended for research-use quantification of human CRP in validated sample types.

Applications

  • Quantification of human CRP / C-reactive protein
  • Inflammation and acute-phase response research
  • Immunology research
  • Infectious disease research
  • Autoimmune and chronic inflammatory disease research
  • Cardiovascular research
  • Cancer and tissue-damage related research applications

Assay principle

The assay uses a solid-phase sandwich ELISA format. Samples, standards or controls are added to wells pre-coated with a target-specific capture antibody. Human CRP present in the sample binds to the immobilized antibody and is detected using the supplied detection reagents. The resulting colorimetric signal is measured with a microplate reader and is proportional to the amount of CRP in the original sample.

Specifications

  • Protein name: C-reactive protein
  • Alternative names: C Reactive Protein; C-reactive protein (1-205); C-reactive protein, pentraxin-related; CRP; pentraxin 1; PTX1
  • Species reactivity: Human
  • Assay format: Solid-phase sandwich ELISA, quantitative
  • Sample type: serum, plasma and cell culture supernatant
  • Detection method: Colorimetric
  • Instrument: Microplate reader
  • UniProt ID: P02741
  • Recovery: serum 98.9%, plasma 90.7%, cell culture media 93.1%
Targets:
CRP
Research Area:
Acute Phase Response • Autoimmune Disease Research • Cancer Research • Cardiovascular Research • Immunology • Infectious Disease Research • Inflammation
Application:
ELISA
Reactivity:
Human
Specificity:
Human CRP / C-reactive protein
Formulation:
Complete 96-well solid-phase sandwich ELISA kit with pre-coated breakable strip microplate and assay reagents
UniProt:
P02741
Storage Temperature:
4°C

For Research Use Only. Not intended for diagnostic or therapeutic use.
Products may be subject to intellectual property rights.

The purchase of this product does not grant any license for commercial use, manufacturing, or clinical applications. The user is responsible for ensuring compliance with applicable laws and third-party rights.