IGF1R (IGFR or CD221) Antibody
BiCell Scientific
SKU:52091-50UL
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Protein Function
This antibody helps you analyze IGF1R (IGFR) while keeping condition-to-condition contrasts straightforward. IGF1R is a pathway component whose activation state and localization can change after stimulation. Use matched handling so differences reflect biology rather than processing.
Monitoring IGF1R alongside context markers can improve interpretation when phenotypes are subtle.
Experimental Applications
When comparing conditions, consistent sample handling and normalization are key for meaningful contrasts. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- condition-to-condition comparisons with matched controls
- localization shifts between cytoplasm, membrane, and nucleus
- interaction patterns within multi-protein complexes
- time-resolved changes following stimulation or inhibition
Workflow Perspective
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so IGF1R patterns can be compared across experiments without guesswork.
Pair this regular format format with stable controls to keep IGF1R (IGFR) measurements coherent over time.
- Targets:
- IGF1R
- Research Area:
- Cell Signaling • Developmental Biology • Endocrinology • Immunology • Metabolism
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal region of mouse IGF1R protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P08069
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 93.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C