Indirubin  (NSC 105327)

Indirubin  (NSC 105327) is an inhibitor of GSK-3 used in studies of PI3K-Akt-mTOR Signaling and Wnt Signaling. In practice, this places the compound in experiments that measure growth-factor signaling, survival control, and nutrient-sensing outputs and beta-catenin-dependent transcription and developmental signaling in cancer, cell signaling, and developmental biology models.

By inhibiting GSK-3, Indirubin  (NSC 105327) can be used to examine growth-factor signaling, survival control, and nutrient-sensing outputs and beta-catenin-dependent transcription and developmental signaling. The kinase annotation adds relevance to biochemical kinase assays, phospho-signaling studies, and selectivity profiling, together with downstream-response mapping in the same experimental setting. In cancer, cell signaling, and developmental biology models, these readouts can be combined with viability, reporter, localization, biochemical conversion, or morphology endpoints to refine experimental interpretation.

Research Applications

• Target-focused assays involving GSK-3
• Pathway perturbation studies connected to PI3K-Akt-mTOR Signaling and Wnt Signaling
• Concentration-response inhibition and target-dependence studies
• Biochemical kinase assays, phospho-signaling studies, and selectivity profiling

Overall, Indirubin  (NSC 105327) is appropriate when a defined chemical perturbant is needed to connect GSK-3 with measurable biochemical, transcriptional, electrophysiological, imaging, or phenotypic readouts in cancer, cell signaling, and developmental biology models. This profile is suited to mechanistic follow-up, comparative profiling, and assay optimization under defined exposure conditions.