Integrin beta-3 (ITGB3 or CD61) Antibody - FFPE Validated

Biological Background

This antibody supports experimental designs that analyze Integrin beta-3 (CD61) with interpretable controls and clear contrasts. Integrin beta-3 is an adhesion-related target that can shift with substrate composition and mechanical cues. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol CD61.

Use in the Laboratory

Perturbations that disrupt integrin engagement or actomyosin tension can reshape localization and complex assembly. Time course design can be decisive when regulation is transient or compartment dependent.

Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.

To keep datasets interpretable, labs often document:

• focal adhesion versus diffuse membrane distribution
• co-variation with cytoskeletal and scaffold partners
• remodeling after changes in substrate or mechanical stress

Version Notes

For paraffin-embedded samples, fixation history and retrieval strategy matter; this option is aligned to those constraints. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Integrin beta-3 patterns can be compared across experiments without guesswork.

The fixed-section format fits designs where Integrin beta-3 (CD61) is evaluated with deliberate controls and careful comparisons.