Integrin beta-4 (ITGB4 or CD104) Antibody - Western Blot Validated

Biological Background

This antibody supports experimental designs that investigate Integrin beta-4 (ITGB4) with interpretable controls and clear contrasts. Integrin beta-4 is an extracellular or receptor-linked component that coordinates matrix engagement with cytoskeletal remodeling. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol ITGB4.

Use in the Laboratory

Comparative experiments can separate expression differences from changes in adhesion structure abundance. Time course design can be decisive when regulation is transient or compartment dependent.

Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.

To keep datasets interpretable, labs often document:

• focal adhesion versus diffuse membrane distribution
• remodeling after changes in substrate or mechanical stress
• effects of adhesion inhibitors or matrix composition

Version Notes

If your primary readout depends on denatured protein presentation, this option can streamline comparison across lanes and conditions. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

If your study spans multiple model systems, it can help to define a shared reference condition for Integrin beta-4 so cross-sample contrasts stay meaningful.

If your study spans multiple model systems, it can help to define a shared reference condition for Integrin beta-4 so cross-sample contrasts stay meaningful.

Use the denaturing-format option to keep Integrin beta-4 (ITGB4) analysis aligned with planned contrasts and endpoints.