JAG2 Antibody - FFPE Validated
BiCell Scientific
SKU:51507-FFPE-50UL
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About the Target
For consistent comparison across experiments, use this antibody to characterize JAG2 with a repeatable workflow. JAG2 is a signaling node often evaluated by abundance, localization, and modification-dependent states. Use matched handling so differences reflect biology rather than processing.
Following JAG2 across perturbations can help separate expression effects from compartment shifts.
Research Context
When comparing conditions, consistent sample handling and normalization are key for meaningful contrasts. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Consider these angles when interpreting target-level changes:
- interaction patterns within multi-protein complexes
- time-resolved changes following stimulation or inhibition
- localization shifts between cytoplasm, membrane, and nucleus
- condition-to-condition comparisons with matched controls
Variant Considerations
Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.
For challenging targets, pairing JAG2 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
If your study spans multiple model systems, it can help to define a shared reference condition for JAG2 so cross-sample contrasts stay meaningful.
Pair this fixed-section format with stable controls to keep JAG2 measurements coherent over time.
- Targets:
- JAG2
- Research Area:
- Cell Signaling • Developmental Biology
- Application:
- IHC-P
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal cytoplasmic region of mouse JAG2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9Y219
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 77.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C