Ki67 (C-Terminus) Antibody
BiCell Scientific
SKU:53100-50UL
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Protein Function
This antibody helps you evaluate Ki67 C-Terminus while keeping condition-to-condition contrasts straightforward. Ki67 C-Terminus is a molecular target often examined to connect phenotype changes with underlying protein regulation. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Monitoring Ki67 C-Terminus alongside context markers can improve interpretation when phenotypes are subtle.
Experimental Applications
Temporal sampling and matched controls can improve interpretation when expression is dynamic. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies. Stem or progenitor models may incorporate this target when mapping state transitions and lineage drift.
Common experimental questions that benefit from target tracking include:
- subcellular compartment mapping
- cross-sample normalization strategies
- interpretation alongside orthogonal readouts
- baseline versus stimulated condition comparisons
Workflow Perspective
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Ki67 C-Terminus patterns can be compared across experiments without guesswork.
For multi-run studies, the regular format option helps keep Ki67 C-Terminus trends comparable from experiment to experiment.
- Targets:
- MKI67
- Research Area:
- Cell Cycle • Stem Cell Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (13-aa) derived from the C-terminal region of mouse Ki67 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P46013
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 76.9% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C