KLF4 Antibody
BiCell Scientific
SKU:53144-50UL
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About the Target
When control selection matters, this antibody helps you assess KLF4 in a structured study. KLF4 is a protein of interest for mapping abundance changes across conditions and cell states. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
Tracking KLF4 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Research Context
Comparative experiments across cell states can uncover patterns that single-condition measurements miss. Time course design can be decisive when regulation is transient or compartment dependent. Stem or progenitor models may incorporate this target when mapping state transitions and lineage drift.
Consider these angles when interpreting target-level changes:
- cross-sample normalization strategies
- baseline versus stimulated condition comparisons
- subcellular compartment mapping
Variant Considerations
This regular format is a practical starting point when you want flexibility across sample types and iterative optimization. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.
For challenging targets, pairing KLF4 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
If your study spans multiple model systems, it can help to define a shared reference condition for KLF4 so cross-sample contrasts stay meaningful.
Integrate the regular format version into your design so KLF4 signals can be interpreted alongside matched controls.
- Targets:
- KLF4
- Research Area:
- Stem Cell Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (13-aa) derived from the C-terminal region of human KLF4 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- O43474
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C