LCK (LSK) Antibody
BiCell Scientific
SKU:52417-50UL
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Biological Background
This antibody enables you to explore LCK with a direct, biology-first readout. LCK is a regulator within kinase-driven cascades that influence transcriptional and metabolic responses. Look for shifts in distribution that track with phenotype changes.
Following LCK across perturbations can help separate expression effects from compartment shifts.
Use in the Laboratory
Signaling targets are typically assessed across time courses to capture transient activation windows. Pair measurements with orthogonal readouts when interpretation is sensitive to context. In oncology research models, expression shifts can accompany changes in adhesion, signaling, or differentiation state.
Depending on the model, researchers may ask whether signal shifts reflect altered abundance, compartment changes, or remodeling of associated complexes.
To keep datasets interpretable, labs often document:
- condition-to-condition comparisons with matched controls
- localization shifts between cytoplasm, membrane, and nucleus
- interaction patterns within multi-protein complexes
- time-resolved changes following stimulation or inhibition
Version Notes
This regular format is a practical starting point when you want flexibility across sample types and iterative optimization. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.
If your study spans multiple model systems, it can help to define a shared reference condition for LCK so cross-sample contrasts stay meaningful.
When interpreting LCK, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
Select the regular format version to keep LCK interpretation aligned with your workflow goals.
- Targets:
- LCK
- Research Area:
- Cancer • Cell Signaling
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (20-aa) derived from the N-terminal region of mouse LCK protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P06239
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 90% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C