Leptin receptor (LEPR or CD295) Antibody
BiCell Scientific
SKU:52095-50UL
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Biological Background
This antibody supports efforts to explore Leptin receptor (LEPR) and interpret it within interaction networks. Leptin receptor is a signaling protein that can relay information through phosphorylation and complex assembly. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.
Tracking Leptin receptor can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Use in the Laboratory
Perturbations such as inhibitors or ligand stimulation can drive rapid shifts in localization or complex formation. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution.
Depending on the model, researchers may ask whether signal shifts reflect altered abundance, compartment changes, or remodeling of associated complexes.
To keep datasets interpretable, labs often document:
- time-resolved changes following stimulation or inhibition
- localization shifts between cytoplasm, membrane, and nucleus
- interaction patterns within multi-protein complexes
Version Notes
If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.
When interpreting Leptin receptor, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
Choose the regular format format to study Leptin receptor (LEPR) in a way that stays anchored to pathway logic.
- Targets:
- LEPR
- Research Area:
- Cell Signaling • Developmental Biology • Endocrinology • Immunology • Metabolism
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (13-aa) derived from the C-terminal cytoplasmic region of human LEPR protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P48357
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C