LGR5 (GPR49 or GPR67) Antibody - FFPE Validated

Scientific Overview

This antibody helps you analyze LGR5 (GPR49) while keeping condition-to-condition contrasts straightforward. LGR5 is a signaling protein that can relay information through phosphorylation and complex assembly. Use matched handling so differences reflect biology rather than processing.

In some datasets and protocols, it is referenced by the gene symbol GPR49.

Research Relevance

Parallel measurement of upstream inputs and downstream readouts can sharpen interpretation of pathway behavior. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood. Stem or progenitor models may incorporate this target when mapping state transitions and lineage drift.

When building a narrative around the target, it helps to track:

• time-resolved changes following stimulation or inhibition
• localization shifts between cytoplasm, membrane, and nucleus
• condition-to-condition comparisons with matched controls
• interaction patterns within multi-protein complexes

Application-Specific Context

Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.

When interpreting LGR5, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

For multi-run studies, the fixed-section option helps keep LGR5 (GPR49) trends comparable from experiment to experiment.