LLGL2 (LGL2) Antibody - Western Blot Validated
BiCell Scientific
SKU:00272-WB-50UL
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Protein Function
Designed to help you investigate LLGL2, this antibody supports studies where protein context matters. LLGL2 is a signaling node often evaluated by abundance, localization, and modification-dependent states. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
Following LLGL2 across perturbations can help separate expression effects from compartment shifts.
Experimental Applications
Perturbations such as inhibitors or ligand stimulation can drive rapid shifts in localization or complex formation. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- time-resolved changes following stimulation or inhibition
- localization shifts between cytoplasm, membrane, and nucleus
- condition-to-condition comparisons with matched controls
Workflow Perspective
When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
When interpreting LLGL2, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so LLGL2 patterns can be compared across experiments without guesswork.
The denaturing-format version supports deeper biological narratives around LLGL2 without overcomplicating setup.
- Targets:
- LLGL2
- Research Area:
- Cell Signaling • Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (14-aa) derived from the C-terminal region of mouse LLGL2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q6P1M3
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 92.9% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C