MAGI2 (AIP1 or NPHS15) Antibody - FFPE Validated

About the Target

Plan your comparisons around MAGI2 (AIP1) and use this antibody to examine state-dependent differences. MAGI2 is a junctional protein that coordinates adhesion with cytoskeletal anchoring. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol AIP1.

Research Context

Junction proteins are frequently analyzed during differentiation, stress responses, and changes in cell polarity. Time course design can be decisive when regulation is transient or compartment dependent. Kidney disease modeling efforts may include this target when exploring podocyte stress responses and structural change.

Consider these angles when interpreting target-level changes:

• changes in complex assembly with scaffold or adaptor proteins
• subcellular redistribution at contact sites versus cytoplasm
• co-localization patterns with cytoskeletal elements

Variant Considerations

Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so MAGI2 patterns can be compared across experiments without guesswork.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so MAGI2 patterns can be compared across experiments without guesswork.

Use the fixed-section option to keep MAGI2 (AIP1) analysis aligned with planned contrasts and endpoints.