MBD1 Antibody - Western Blot Validated
BiCell Scientific
SKU:82011-WB-50UL
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Biological Background
Use this antibody to investigate MBD1 in your model system. MBD1 is a target that supports characterization of cellular state through expression and localization readouts. Look for shifts in distribution that track with phenotype changes.
Following MBD1 across perturbations can help separate expression effects from compartment shifts.
Use in the Laboratory
Comparative experiments across cell states can uncover patterns that single-condition measurements miss. Pair measurements with orthogonal readouts when interpretation is sensitive to context.
Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.
To keep datasets interpretable, labs often document:
- cross-sample normalization strategies
- baseline versus stimulated condition comparisons
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
Version Notes
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so MBD1 patterns can be compared across experiments without guesswork.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so MBD1 patterns can be compared across experiments without guesswork.
If your study spans multiple model systems, it can help to define a shared reference condition for MBD1 so cross-sample contrasts stay meaningful.
With a clear focus on MBD1, this denaturing-format option fits streamlined studies and fast iteration.
- Targets:
- MBD1
- Research Area:
- Epigenetics
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of mouse MBD1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9UIS9
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 88.2% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C