MBD3 Antibody - FFPE Validated

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BiCell Scientific

SKU:82013-FFPE-50UL

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Size
Blocking Peptide
Quantity
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Protein Function

Use this antibody to investigate MBD3 when mapping complex behavior and signaling context. MBD3 is a cellular protein that can be tracked to understand expression patterns and compartmental behavior. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.

Following MBD3 across perturbations can help separate expression effects from compartment shifts.

Experimental Applications

Integrating localization with abundance measurements can clarify whether changes reflect trafficking or regulation. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution.

Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.

Common experimental questions that benefit from target tracking include:

  • subcellular compartment mapping
  • baseline versus stimulated condition comparisons
  • interpretation alongside orthogonal readouts

Workflow Perspective

For paraffin-embedded samples, fixation history and retrieval strategy matter; this option is aligned to those constraints. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. If interactions are a focus, consider parallel readouts for key partners.

When interpreting MBD3, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so MBD3 patterns can be compared across experiments without guesswork.

Choose the fixed-section format to study MBD3 in a way that stays anchored to pathway logic.

Targets:
MBD3
Research Area:
Epigenetics
Application:
IHC-P
Reactivity:
Human • Mouse • Rat
Host:
Rat
Clonality:
Polyclonal
Isotype:
IgG
Immunogen:
Synthetic peptide (14-aa) derived from the N-terminal region of human MBD3 protein
Conjugation:
Unconjugated
Purification:
Affinity Chromatography
Concentration:
0.25 mg/ml
UniProt:
O95983
Homology:
Synthetic peptide sequence is identical to mouse or rat sequence
Storage Buffer:
PBS, pH 7.2, 0.1% Sodium Azide
Storage Temperature:
-20°C