Phospho-Met/c-Met (Tyr1349) Antibody

About the Target

MET/C-met is a target of interest in many antibody-based workflows. Gab1 is a type of docking protein that belongs to a group of similar proteins, including IRS, FRS-2/SNT1, and p62dok. These proteins are substrates of tyrosine kinases and contain an NH2-terminal lipid-binding domain, such as a PH domain or NH2-terminal myristylation sequence, for membrane targeting and a central PTB-like domain for association with specific tyrosine kinases. Depending on the literature source, MET/C-met may also be discussed as Phospho-Met/c-Met (Tyr1349) and Phospho Met (Tyr 1349 ).

Reported cellular context includes membrane, cell membrane, and secreted, which can matter when signal is compared across treatments or changing cell states. Following MET/C-met across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

MET/C-met is commonly interpreted in the context of cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans membrane, cell membrane, and secreted, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between membrane, cell membrane, and secreted across matched conditions
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation
• differences between total target abundance and site-specific regulation when modified forms are compared
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for MET/C-met. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in MET/C-met reflect biology rather than handling. When interpreting MET/C-met, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep MET/C-met trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.