Metallothionein-2 (MT2 or MT2A) (Human) Antibody - Western Blot Validated
BiCell Scientific
SKU:10312-WB-50UL
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Protein Function
This antibody supports experimental designs that analyze Metallothionein-2 with interpretable controls and clear contrasts. Metallothionein-2 is a protein of interest for mapping abundance changes across conditions and cell states. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
Monitoring Metallothionein-2 alongside context markers can improve interpretation when phenotypes are subtle.
Experimental Applications
Temporal sampling and matched controls can improve interpretation when expression is dynamic. Time course design can be decisive when regulation is transient or compartment dependent.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.
Common experimental questions that benefit from target tracking include:
- baseline versus stimulated condition comparisons
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
Workflow Perspective
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.
If your study spans multiple model systems, it can help to define a shared reference condition for Metallothionein-2 so cross-sample contrasts stay meaningful.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Metallothionein-2 patterns can be compared across experiments without guesswork.
Integrate the denaturing-format version into your design so Metallothionein-2 signals can be interpreted alongside matched controls.
- Targets:
- MT2A
- Research Area:
- Apoptosis • Metabolism • Oxidative Stress
- Application:
- WB
- Reactivity:
- Human
- Host:
- Mouse
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (21-aa) derived from the N-terminal region of human Metallothionein-2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P02795
- Homology:
- Synthetic peptide sequence showing 71.4% homology to rodent sequences
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C