Myc (c-Myc) Antibody - FFPE Validated
BiCell Scientific
SKU:53105-FFPE-50UL
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Biological Background
For consistent comparison across experiments, use this antibody to examine Myc (c-Myc) with a repeatable workflow. Myc is a protein of interest for mapping abundance changes across conditions and cell states. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
In some datasets and protocols, it is referenced by the gene symbol c-Myc.
Use in the Laboratory
Temporal sampling and matched controls can improve interpretation when expression is dynamic. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows. In oncology research models, expression shifts can accompany changes in adhesion, signaling, or differentiation state.
Depending on the model, researchers may ask whether signal shifts reflect altered abundance, compartment changes, or remodeling of associated complexes.
To keep datasets interpretable, labs often document:
- baseline versus stimulated condition comparisons
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
- cross-sample normalization strategies
Version Notes
Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. A brief pilot run can help set exposure ranges and reduce reruns later.
For challenging targets, pairing Myc measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Pair this fixed-section format with stable controls to keep Myc (c-Myc) measurements coherent over time.
- Targets:
- MYC
- Research Area:
- Cancer • Cell Cycle • Cell Signaling • Stem Cell Biology
- Application:
- IHC-P
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal region of mouse Myc protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P01106
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 93.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C