NEPH1 (KIRREL1) (N-Terminus) Antibody - Western Blot Validated
BiCell Scientific
SKU:00177N-WB-50UL
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Protein Function
When you need to analyze NEPH1 N-Terminus (KIRREL1) with attention to cellular organization, this antibody provides a focused option. NEPH1 N-Terminus is a contact-site protein that shapes barrier properties and signaling at the cell surface. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
Tracking NEPH1 N-Terminus can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Junction proteins are frequently analyzed during differentiation, stress responses, and changes in cell polarity. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
Target-centric readouts can be framed around localization, relative abundance, or complex behavior, depending on the question you are testing.
Common experimental questions that benefit from target tracking include:
- subcellular redistribution at contact sites versus cytoplasm
- comparative profiling across cell states or perturbations
- changes in complex assembly with scaffold or adaptor proteins
Workflow Perspective
When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
If your study spans multiple model systems, it can help to define a shared reference condition for NEPH1 N-Terminus so cross-sample contrasts stay meaningful.
Use the denaturing-format variant when your project demands NEPH1 N-Terminus (KIRREL1) readouts that respect preparation constraints.
- Targets:
- KIRREL1
- Research Area:
- Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the N-terminal extracellular region of human NEPH1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q96J84
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C