NHE2 (Slc9a2) (rat) Antibody - FFPE Validated
BiCell Scientific
SKU:21002RS-FFPE-50UL
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Biological Background
This antibody enables you to explore NHE2 with a direct, biology-first readout. NHE2 is a membrane protein linked to solute movement and gradient maintenance. Focus on trends across conditions rather than single-point snapshots.
In some datasets and protocols, it is referenced by the gene symbol Slc9a2.
Use in the Laboratory
Comparisons across tissues or engineered cell lines can help separate expression effects from localization effects. Pair measurements with orthogonal readouts when interpretation is sensitive to context.
Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.
To keep datasets interpretable, labs often document:
- stimulus-dependent trafficking and turnover
- relative abundance across engineered constructs
- surface versus intracellular pool distribution
- patterns consistent with apical or basolateral sorting
Version Notes
For paraffin-embedded samples, fixation history and retrieval strategy matter; this option is aligned to those constraints. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.
For challenging targets, pairing NHE2 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so NHE2 patterns can be compared across experiments without guesswork.
Select the fixed-section version to keep NHE2 interpretation aligned with your workflow goals.
- Targets:
- SLC9A2
- Research Area:
- Metabolism
- Application:
- IHC-P
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse NHE2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9UBY0
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 75% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C