NOTCH3 Antibody
BiCell Scientific
SKU:51503-50UL
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About the Target
To connect mechanism with measurement, this antibody lets you examine NOTCH3 in pathway-driven studies. NOTCH3 is a signaling node often evaluated by abundance, localization, and modification-dependent states. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.
Following NOTCH3 across perturbations can help separate expression effects from compartment shifts.
Research Context
Signaling targets are typically assessed across time courses to capture transient activation windows. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution. Developmental projects may follow this target across stage-dependent remodeling and tissue patterning.
Consider these angles when interpreting target-level changes:
- interaction patterns within multi-protein complexes
- condition-to-condition comparisons with matched controls
- time-resolved changes following stimulation or inhibition
Variant Considerations
If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. This matters because epitope exposure and background can vary with preparation and extraction steps. If you compare conditions, keep processing steps uniform to support clean interpretation.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so NOTCH3 patterns can be compared across experiments without guesswork.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so NOTCH3 patterns can be compared across experiments without guesswork.
Choose the regular format format to study NOTCH3 in a way that stays anchored to pathway logic.
- Targets:
- NOTCH3
- Research Area:
- Cell Signaling • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse NOTCH3 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9UM47
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 93.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C