Annexin-2/ANXA2 Antibody

About the Target

P36 is a target of interest in many antibody-based workflows. Annexin-2/ANXA2 is a multifunctional, pleiotropic calcium- and anionic, phospholipid-binding protein of the annexin family that exists as a monomer or as a heterotetrameric complex (AIIt) with S100A10. Structurally, ANXA2 contains an N-terminal head domain, which undergoes post-translational modifications (acetylation, phosphorylation) and mediates interactions with S100A10, tissue plasminogen activator (tPA), and other proteins, and a conserved C-terminal core domain composed of four annexin repeats (~70 amino acids each) that bind Ca²⁺, phospholipids, heparin, RNA, and F-actin. Depending on the literature source, P36 may also be discussed as Annexin-2/ANXA2 and ANX2.

Reported cellular context includes basement membrane, extracellular matrix, and secreted, which can matter when signal is compared across treatments or changing cell states. Following P36 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

P36 is commonly interpreted in the context of cancer, neuroscience, and metabolism research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans basement membrane, extracellular matrix, and secreted, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between basement membrane, extracellular matrix, and secreted across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• compartment-specific patterns relevant to neuronal polarity, transport, or synaptic context
• responses linked to nutrient status, mitochondrial state, or metabolic rewiring

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for P36. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in P36 reflect biology rather than handling. When interpreting P36, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep P36 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.