Par6a (PARD6A) Antibody - Western Blot Validated
BiCell Scientific
SKU:00249-WB-50UL
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Biological Background
This antibody is suited for researchers who want to evaluate Par6a (PARD6A) and relate it to cell-state biology. Par6a is a signaling protein that can relay information through phosphorylation and complex assembly. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
In some datasets and protocols, it is referenced by the gene symbol PARD6A.
Use in the Laboratory
Parallel measurement of upstream inputs and downstream readouts can sharpen interpretation of pathway behavior. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.
To keep datasets interpretable, labs often document:
- condition-to-condition comparisons with matched controls
- interaction patterns within multi-protein complexes
- time-resolved changes following stimulation or inhibition
Version Notes
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Small differences in preparation can reshape apparent localization, so interpret results in context.
For challenging targets, pairing Par6a measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
The denaturing-format version supports deeper biological narratives around Par6a (PARD6A) without overcomplicating setup.
- Targets:
- PARD6A
- Research Area:
- Cell Signaling • Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the C-terminal region of mouse Par6a protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9NPB6
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 80% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C