PIK3R1 (p85 or p85alpha) Antibody
BiCell Scientific
SKU:52761-50UL
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Scientific Overview
Designed to help you characterize PIK3R1, this antibody supports studies where protein context matters. PIK3R1 is a signaling protein that can relay information through phosphorylation and complex assembly. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
In some datasets and protocols, it is referenced by the gene symbol p85.
Research Relevance
Parallel measurement of upstream inputs and downstream readouts can sharpen interpretation of pathway behavior. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood.
When building a narrative around the target, it helps to track:
- time-resolved changes following stimulation or inhibition
- interaction patterns within multi-protein complexes
- localization shifts between cytoplasm, membrane, and nucleus
Application-Specific Context
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so PIK3R1 patterns can be compared across experiments without guesswork.
When interpreting PIK3R1, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
As you refine hypotheses around PIK3R1, the regular format format helps keep conclusions tied to preparation context.
- Targets:
- PIK3R1
- Research Area:
- Cell Signaling • Metabolism
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the C-terminal region of human PIK3R1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P27986
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C