PI3 Kinase p55 Antibody

About the Target

PIK3R3 is a target of interest in many antibody-based workflows. PI3K p55 is a regulatory subunit of class IA phosphoinositide 3-kinases (PI3Ks), derived from alternative splicing of the PIK3R1 gene, which also produces the p85 subunit. Structurally, p55 is a 55-kDa protein, contains a unique 30-residue N-terminus, a proline-rich motif, and two Src homology 2 (SH2) domains similar to those in p85. Depending on the literature source, PIK3R3 may also be discussed as PI3 Kinase p55.

Reported cellular context includes cytosol, which can matter when signal is compared across treatments or changing cell states. Following PIK3R3 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

PIK3R3 is commonly interpreted in the context of cancer and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytosol, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• signal enrichment within cytosol relative to the broader cellular background
• changes associated with proliferative state, oncogenic signaling, or treatment response
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for PIK3R3. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in PIK3R3 reflect biology rather than handling. When interpreting PIK3R3, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep PIK3R3 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.