PIK3R4 (p150) Antibody

Biological Background

This antibody supports experimental designs that investigate PIK3R4 with interpretable controls and clear contrasts. PIK3R4 is a signaling protein that can relay information through phosphorylation and complex assembly. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol p150.

Use in the Laboratory

Signaling targets are typically assessed across time courses to capture transient activation windows. Time course design can be decisive when regulation is transient or compartment dependent.

Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.

To keep datasets interpretable, labs often document:

• time-resolved changes following stimulation or inhibition
• condition-to-condition comparisons with matched controls
• interaction patterns within multi-protein complexes

Version Notes

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

For challenging targets, pairing PIK3R4 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so PIK3R4 patterns can be compared across experiments without guesswork.

Use the regular format option to keep PIK3R4 analysis aligned with planned contrasts and endpoints.