Podocalyxin (PODXL or gp135) Antibody - Western Blot Validated

Biological Background

When control selection matters, this antibody helps you assess Podocalyxin in a structured study. Podocalyxin is a membrane-associated component that helps organize cell-cell contacts and polarity. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol PODXL.

Use in the Laboratory

Junction proteins are frequently analyzed during differentiation, stress responses, and changes in cell polarity. Time course design can be decisive when regulation is transient or compartment dependent. Podocyte biology studies may assess this target when examining slit-diaphragm organization and cytoskeletal coupling.

Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.

To keep datasets interpretable, labs often document:

• changes in complex assembly with scaffold or adaptor proteins
• co-localization patterns with cytoskeletal elements
• subcellular redistribution at contact sites versus cytoplasm

Version Notes

For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

If your study spans multiple model systems, it can help to define a shared reference condition for Podocalyxin so cross-sample contrasts stay meaningful.

Integrate the denaturing-format version into your design so Podocalyxin signals can be interpreted alongside matched controls.