Spinophilin Antibody

About the Target

Spinophilin, also known as PPP1R9B and NEURABIN-2, is a multifunctional scaffold protein located on chromosome 17. It is enriched in dendritic spines, facilitating synaptic plasticity by anchoring protein phosphatase-1 (PP-1) to ionotropic glutamate receptors such as AMPA and NMDA receptors via its PDZ domain. This interaction regulates glutamatergic synaptic transmission and influences dendritic spine morphology, contributing to the dynamic remodeling of the actin cytoskeleton in neurons. Depending on the literature source, PPP1R9B may also be discussed as Spinophilin.

Reported cellular context includes cell junction, cell membrane, cell projection, and cytoplasm, which can matter when signal is compared across treatments or changing cell states. Following PPP1R9B across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

PPP1R9B is commonly interpreted in the context of cancer, neuroscience, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell junction, cell membrane, and cell projection, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell junction, cell membrane, and cell projection across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• compartment-specific patterns relevant to neuronal polarity, transport, or synaptic context
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for PPP1R9B. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in PPP1R9B reflect biology rather than handling. When interpreting PPP1R9B, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep PPP1R9B trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.