Phospho-PRC1 (Thr481) Antibody

About the Target

Phospho-PRC1 (Thr481) refers to the phosphorylated form of the microtubule-binding protein PRC1 at threonine 481, a key regulatory site within its C-terminal region. PRC1 is crucial for bundling antiparallel microtubules at the spindle midzone during cell division, and its activity is tightly regulated: during early mitosis, Cdk1/cyclin B phosphorylates PRC1 at Thr481, inhibiting its bundling function to prevent premature spindle stabilization; as cells progress to anaphase and Cdk1 activity declines, dephosphorylation at Thr481 reactivates PRC1, enabling proper spindle midzone formation and successful cytokinesis. Depending on the literature source, PRC1 may also be discussed as Phospho-PRC1 (Thr481) and Protein regulator of cytokinesis 1.

Reported cellular context includes chromosome, cytoplasm, cytoskeleton, and microtubule, which can matter when signal is compared across treatments or changing cell states. Following PRC1 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

PRC1 is commonly interpreted in the context of cell cycle research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans chromosome, cytoplasm, and cytoskeleton, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between chromosome, cytoplasm, and cytoskeleton across matched conditions
• cell-cycle linked differences in abundance, timing, or compartmental enrichment
• differences between total target abundance and site-specific regulation when modified forms are compared
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for PRC1. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in PRC1 reflect biology rather than handling. When interpreting PRC1, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep PRC1 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.