RASSF1a Antibody

About the Target

RASSF1A (Ras association domain family 1 isoform A) is a tumor suppressor protein frequently inactivated in human cancers, primarily through epigenetic silencing via promoter methylation. RASSF1A contains a Ras association (RA) domain and a cysteine-rich domain (CRD), which enable interactions with Ras proteins and other signalling molecules.

Reported cellular context includes cytoplasm, cytoskeleton, microtubule, and nucleus, which can matter when signal is compared across treatments or changing cell states. Following RASSF1A across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

RASSF1A is commonly interpreted in the context of cancer, cell cycle, and apoptosis research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm, cytoskeleton, and microtubule, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cytoplasm, cytoskeleton, and microtubule across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• cell-cycle linked differences in abundance, timing, or compartmental enrichment
• separation of survival-associated changes from stress or death-associated readouts

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for RASSF1A. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in RASSF1A reflect biology rather than handling. When interpreting RASSF1A, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep RASSF1A trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.