Rhodamine 123
Selleck Chemicals
SKU:S3577-5MG
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Rhodamine 123 is an inhibitor of ATPases used in studies of enzyme regulation. In practice, this places the compound in experiments that measure catalytic activity, substrate turnover, and enzymatic regulation in cell signaling models. Dose-response comparison, orthogonal validation, and secondary assay follow-up can further sharpen mechanistic interpretation.
By inhibiting ATPases, Rhodamine 123 can be used to examine catalytic activity, substrate turnover, and enzymatic regulation. This target class is commonly examined through enzyme-activity assays, substrate-conversion studies, and mechanism profiling, together with downstream-response mapping. In cell signaling models, these readouts can be combined with viability, reporter, localization, biochemical conversion, or morphology endpoints to refine experimental interpretation.
Research Applications
- Target-focused assays involving ATPases
- Concentration-response inhibition and target-dependence studies
- Enzyme-activity assays, substrate-conversion studies, and mechanism profiling
- Phenotypic profiling in cell signaling models
Overall, Rhodamine 123 is appropriate when a defined chemical perturbant is needed to connect ATPases with measurable biochemical, transcriptional, electrophysiological, imaging, or phenotypic readouts in cell signaling models. This profile is suited to mechanistic follow-up, comparative profiling, and assay optimization under defined exposure conditions.
- Targets:
- ATPases
- Target Class:
- Enzyme
- Research Area:
- Cell Signaling
- CAS No.:
- 62669-70-9
- Molecular Weight:
- 380.82
- SMILES:
- Cl.COC(=O)C1=CC=CC=C1C2=C3C=CC(=N)C=C3OC4=C2C=CC(=C4)N
- Storage Temperature:
- -20°C
For Research Use Only. Not intended for diagnostic or therapeutic use.
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