ROBO2 Antibody
BiCell Scientific
SKU:50832-50UL
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Protein Function
Build reproducible readouts by using this antibody to examine ROBO2 across matched sample sets. ROBO2 is a receptor or synapse-associated protein that supports neuronal signaling and connectivity. Use matched handling so differences reflect biology rather than processing.
Following ROBO2 across perturbations can help separate expression effects from compartment shifts.
Experimental Applications
Perturbations that alter activity or trafficking can shift localization between soma, neurites, and synaptic regions. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Target-centric readouts can be framed around localization, relative abundance, or complex behavior, depending on the question you are testing. Neural differentiation and synaptic organization projects may include this target as a contextual readout.
Common experimental questions that benefit from target tracking include:
- activity-linked changes in abundance or distribution
- comparison across differentiation time courses
- interaction tendencies with scaffold or receptor partners
- regional localization along neurites and synaptic compartments
Workflow Perspective
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
If your study spans multiple model systems, it can help to define a shared reference condition for ROBO2 so cross-sample contrasts stay meaningful.
Pair this regular format format with stable controls to keep ROBO2 measurements coherent over time.
- Targets:
- ROBO2
- Research Area:
- Cell Signaling • Developmental Biology • Neuroscience
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse ROBO2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9HCK4
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 81.3% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C