ROBO4 Antibody - FFPE Validated
BiCell Scientific
SKU:50834-FFPE-50UL
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Protein Function
Designed to help you investigate ROBO4, this antibody supports studies where protein context matters. ROBO4 is a signaling component often monitored in models of neuronal differentiation and plasticity. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
Tracking ROBO4 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Perturbations that alter activity or trafficking can shift localization between soma, neurites, and synaptic regions. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy. Neural differentiation and synaptic organization projects may include this target as a contextual readout.
Common experimental questions that benefit from target tracking include:
- regional localization along neurites and synaptic compartments
- activity-linked changes in abundance or distribution
- interaction tendencies with scaffold or receptor partners
Workflow Perspective
Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so ROBO4 patterns can be compared across experiments without guesswork.
Use the fixed-section variant when your project demands ROBO4 readouts that respect preparation constraints.
- Targets:
- ROBO4
- Research Area:
- Cell Signaling • Developmental Biology • Neuroscience
- Application:
- IHC-P
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse ROBO4 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q8WZ75
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 87.5% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C