ROBO4 Antibody - Western Blot Validated
BiCell Scientific
SKU:50834-WB-50UL
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About the Target
Bring ROBO4 into view to characterize changes across conditions. ROBO4 is a signaling component often monitored in models of neuronal differentiation and plasticity. Focus on trends across conditions rather than single-point snapshots.
Following ROBO4 across perturbations can help separate expression effects from compartment shifts.
Research Context
In mixed cultures, cell-type composition can influence apparent signal, so normalization strategies matter. Pair measurements with orthogonal readouts when interpretation is sensitive to context. Neural differentiation and synaptic organization projects may include this target as a contextual readout.
Consider these angles when interpreting target-level changes:
- comparison across differentiation time courses
- interaction tendencies with scaffold or receptor partners
- regional localization along neurites and synaptic compartments
- activity-linked changes in abundance or distribution
Variant Considerations
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. This matters because epitope exposure and background can vary with preparation and extraction steps. If you compare conditions, keep processing steps uniform to support clean interpretation.
For challenging targets, pairing ROBO4 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
For challenging targets, pairing ROBO4 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Select the denaturing-format version to keep ROBO4 interpretation aligned with your workflow goals.
- Targets:
- ROBO4
- Research Area:
- Cell Signaling • Developmental Biology • Neuroscience
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse ROBO4 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q8WZ75
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 87.5% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C
For Research Use Only. Not intended for diagnostic or therapeutic use.
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The purchase of this product does not grant any license for commercial use, manufacturing, or clinical applications. The user is responsible for ensuring compliance with applicable laws and third-party rights.