SGLT2 (Slc5a2) (Human) Antibody - Western Blot Validated
BiCell Scientific
SKU:20802H-WB-50UL
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Protein Function
Designed to help you analyze SGLT2 (Slc5a2), this antibody supports studies where protein context matters. SGLT2 is a channel or transporter associated with regulated flux across cellular membranes. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
In some datasets and protocols, it is referenced by the gene symbol Slc5a2.
Experimental Applications
Many transport proteins cycle through endosomal pathways, making localization a key readout alongside abundance. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- surface versus intracellular pool distribution
- stimulus-dependent trafficking and turnover
- relative abundance across engineered constructs
Workflow Perspective
When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so SGLT2 patterns can be compared across experiments without guesswork.
When interpreting SGLT2, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
The denaturing-format version supports deeper biological narratives around SGLT2 (Slc5a2) without overcomplicating setup.
- Targets:
- SLC5A2
- Research Area:
- Cell Signaling • Metabolism
- Application:
- WB
- Reactivity:
- Human
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the N-terminal cytoplasmic region of human Sglt2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P31639
- Homology:
- Synthetic peptide sequence showing 62.5% homology to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C