SLC12A8 (CCC9) (Zebrafish) Antibody - Western Blot Validated
BiCell Scientific
SKU:20308Z-WB-50UL
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About the Target
When control selection matters, this antibody helps you evaluate SLC12A8 in a structured study. SLC12A8 is a membrane protein linked to solute movement and gradient maintenance. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
In some datasets and protocols, it is referenced by the gene symbol CCC9.
Research Context
Transport targets are commonly studied under stimulation conditions that alter membrane potential, osmolarity, or nutrient availability. Time course design can be decisive when regulation is transient or compartment dependent.
Consider these angles when interpreting target-level changes:
- patterns consistent with apical or basolateral sorting
- stimulus-dependent trafficking and turnover
- surface versus intracellular pool distribution
Variant Considerations
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.
When interpreting SLC12A8, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so SLC12A8 patterns can be compared across experiments without guesswork.
Integrate the denaturing-format version into your design so SLC12A8 signals can be interpreted alongside matched controls.
- Targets:
- SLC12A8
- Research Area:
- Metabolism
- Application:
- WB
- Reactivity:
- Zebrafish
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal cytoplasmic region of zebrafish SLC12A8 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- Homology:
- Synthetic peptide sequence showing <50% homology to human sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C