SLIT2 Antibody - Western Blot Validated
BiCell Scientific
SKU:50842-WB-50UL
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Biological Background
For consistent comparison across experiments, use this antibody to assess SLIT2 with a repeatable workflow. SLIT2 is a neuron-enriched target connected to synaptic organization and signaling dynamics. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Tracking SLIT2 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Use in the Laboratory
Because many neuronal proteins form complexes, interpreting signal changes can benefit from complementary controls and timing. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows. Neural differentiation and synaptic organization projects may include this target as a contextual readout.
Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.
To keep datasets interpretable, labs often document:
- regional localization along neurites and synaptic compartments
- comparison across differentiation time courses
- activity-linked changes in abundance or distribution
- interaction tendencies with scaffold or receptor partners
Version Notes
If your primary readout depends on denatured protein presentation, this option can streamline comparison across lanes and conditions. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. A brief pilot run can help set exposure ranges and reduce reruns later.
For challenging targets, pairing SLIT2 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Pair this denaturing-format format with stable controls to keep SLIT2 measurements coherent over time.
- Targets:
- SLIT2
- Research Area:
- Cell Signaling • Developmental Biology • Neuroscience
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of human SLIT2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- O94813
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C