SPRY1 Antibody
BiCell Scientific
SKU:51401-50UL
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About the Target
This antibody helps you evaluate SPRY1 while keeping condition-to-condition contrasts straightforward. SPRY1 is a pathway component whose activation state and localization can change after stimulation. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Monitoring SPRY1 alongside context markers can improve interpretation when phenotypes are subtle.
Research Context
Perturbations such as inhibitors or ligand stimulation can drive rapid shifts in localization or complex formation. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows. Developmental projects may follow this target across stage-dependent remodeling and tissue patterning.
Consider these angles when interpreting target-level changes:
- localization shifts between cytoplasm, membrane, and nucleus
- interaction patterns within multi-protein complexes
- time-resolved changes following stimulation or inhibition
- condition-to-condition comparisons with matched controls
Variant Considerations
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.
If your study spans multiple model systems, it can help to define a shared reference condition for SPRY1 so cross-sample contrasts stay meaningful.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so SPRY1 patterns can be compared across experiments without guesswork.
Pair this regular format format with stable controls to keep SPRY1 measurements coherent over time.
- Targets:
- SPRY1
- Research Area:
- Cell Signaling • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (14-aa) derived from the internal region of human SPRY1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- O43609
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C