T4 DNA Ligase

Overview

T4 DNA Ligase is a research-use reagent supporting ta cloning workflows. T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in blunt-end or sticky-end dsDNA. Key notes from the supplied features include: >99% purity confirmed by HPLC. The wording is kept close to the supplied product information, helping you reference the right item quickly and document consistently.

Key Benefits

• Quality control: >99% purity confirmed by HPLC.
• nuclease-free componentsReliable performance: Classical products for efficient ligation reactionsComponents.
• T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in blunt-end or sticky-end dsDNA.
• This product also can catalyze the ligation of RNA and ssDNA or RNA strands in double strands, but it cannot catalyze the ligation of whole single-stranded.
• It is suitable for nucleic acid operations such as labeling RNA 3'-ends, circularizing RNA and DNA oligonucleotides or cloning cDNA.

How it supports your workflow

In day-to-day research, teams often need products that fit existing methods while remaining easy to justify in notes, SOPs, and reporting. For ta cloning workflows, the goal is often to keep the scientific rationale and the purchasing record aligned. T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in blunt-end or sticky-end dsDNA. This product also can catalyze the ligation of RNA and ssDNA or RNA strands in double strands, but it cannot catalyze the ligation of whole single-stranded nucleotides. It is suitable for nucleic acid operations such as labeling RNA 3'-ends, circularizing RNA and DNA oligonucleotides or cloning cDNA. The supplied feature set highlights quality control and nuclease-free componentsreliable performance, which can help teams standardize language and expectations across similar products. Because projects evolve, we aim for descriptions that remain searchable, specific, and easy to compare across catalog numbers—so you can reorder confidently and keep documentation consistent.

Applications & compatibility

Use the notes below to gauge fit for your setup and documentation needs. It is suitable for nucleic acid operations such as labeling RNA 3'-ends, circularizing RNA and DNA oligonucleotides or cloning cDNA. If you’re matching this item to an established SOP, use the product name, catalog number, and the supplied features as the primary reference points. Use the application notes as a scope guide: they indicate fit and compatibility language drawn from the supplied product information, not assumptions beyond it.

Ideal for: labs focused on ta cloning workflows; teams that need clear, searchable documentation for purchasing and experimental records; and groups standardizing methods across projects or sites. Ideal when you need a quick, confident match between what’s on the page and what’s in your records.