Tetraspanin-25 (TSPAN25 or CD53) Antibody - FFPE Validated

Scientific Overview

This antibody helps you analyze Tetraspanin-25 while keeping condition-to-condition contrasts straightforward. Tetraspanin-25 is an extracellular or receptor-linked component that coordinates matrix engagement with cytoskeletal remodeling. Use matched handling so differences reflect biology rather than processing.

In some datasets and protocols, it is referenced by the gene symbol CD53.

Research Relevance

Perturbations that disrupt integrin engagement or actomyosin tension can reshape localization and complex assembly. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood.

When building a narrative around the target, it helps to track:

• focal adhesion versus diffuse membrane distribution
• co-variation with cytoskeletal and scaffold partners
• remodeling after changes in substrate or mechanical stress
• effects of adhesion inhibitors or matrix composition

Application-Specific Context

This fixed-section oriented version is intended for paraffin-embedded tissue, where crosslinking and retrieval steps can shape epitope accessibility. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.

For challenging targets, pairing Tetraspanin-25 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

Pair this fixed-section format with stable controls to keep Tetraspanin-25 measurements coherent over time.