TOGARAM1 Antibody - Western Blot Validated
BiCell Scientific
SKU:90704-WB-50UL
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About the Target
For consistent comparison across experiments, use this antibody to evaluate TOGARAM1 with a repeatable workflow. TOGARAM1 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Monitoring TOGARAM1 alongside context markers can improve interpretation when phenotypes are subtle.
Research Context
Disruption of intraflagellar transport or basal body function can produce distinct localization signatures. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Consider these angles when interpreting target-level changes:
- cell-cycle linked differences in localization
- signal enrichment at basal bodies versus cytoplasm
- co-occurrence with intraflagellar transport markers
- changes in cilia incidence or apparent length in perturbation studies
Variant Considerations
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.
If your study spans multiple model systems, it can help to define a shared reference condition for TOGARAM1 so cross-sample contrasts stay meaningful.
When interpreting TOGARAM1, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
Pair this denaturing-format format with stable controls to keep TOGARAM1 measurements coherent over time.
- Targets:
- TOGARAM1
- Research Area:
- Cell Cycle • Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of human TOGARAM1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9Y4F4
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C