TPD52 Antibody

About the Target

TPD52 (Tumor Protein D52) is a proto-oncogene frequently overexpressed in multiple malignancies, including prostate, breast, ovarian, and colorectal cancers, primarily due to gene amplification at the chromosomal locus 8q21. Structurally, TPD52 is a 184-amino acid, hydrophilic protein featuring conserved N- and C-terminal PEST domains-typically linked to protein turnover-alongside a coiled-coil motif critical for protein-protein interactions and a D2 motif essential for functional binding. Depending on the literature source, TPD52 may also be discussed as Tumor protein D52 and Protein N8.

Reported cellular context includes cytoplasm and endoplasmic reticulum, which can matter when signal is compared across treatments or changing cell states. Following TPD52 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

TPD52 is commonly interpreted in the context of cancer and metabolism research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm and endoplasmic reticulum, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cytoplasm and endoplasmic reticulum across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• responses linked to nutrient status, mitochondrial state, or metabolic rewiring
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for TPD52. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in TPD52 reflect biology rather than handling. When interpreting TPD52, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep TPD52 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.