PODXL Antibody

About the Target

TRA-1-60 is a target of interest in many antibody-based workflows. Podocalyxin-like protein (PODXL) is a heavily glycosylated type I transmembrane protein, closely associated with CD34. It is primarily localized in the cytoplasm of tumor cells, sometimes extending towards the cell membrane, but not found within the nucleus. The gene encoding PODXL is situated on chromosome 7q32-q33. Depending on the literature source, TRA-1-60 may also be discussed as PODXL and Podocalyxin.

Reported cellular context includes cell membrane, cell projection, and membrane, which can matter when signal is compared across treatments or changing cell states. Following TRA-1-60 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

TRA-1-60 is commonly interpreted in the context of cancer, neuroscience, and cardiovascular research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane, cell projection, and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell membrane, cell projection, and membrane across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• compartment-specific patterns relevant to neuronal polarity, transport, or synaptic context
• changes linked to vascular, contractile, or hemodynamic cell-state cues

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for TRA-1-60. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in TRA-1-60 reflect biology rather than handling. When interpreting TRA-1-60, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep TRA-1-60 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.