Trpm1 Antibody
BiCell Scientific
SKU:11021-50UL
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Biological Background
Use this antibody to investigate Trpm1 when mapping complex behavior and signaling context. Trpm1 is a membrane-embedded regulator of ionic or osmotic balance in cells. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.
Following Trpm1 across perturbations can help separate expression effects from compartment shifts.
Use in the Laboratory
Modulators that affect trafficking, phosphorylation, or ligand binding can change apparent distribution and signal intensity. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution.
Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.
To keep datasets interpretable, labs often document:
- relative abundance across engineered constructs
- patterns consistent with apical or basolateral sorting
- stimulus-dependent trafficking and turnover
Version Notes
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.
When interpreting Trpm1, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Trpm1 patterns can be compared across experiments without guesswork.
The regular format option supports mechanistic reading of Trpm1 when interactions and context are the focus.
- Targets:
- TRPM1
- Research Area:
- Cell Signaling • Neuroscience
- Application:
- IF • IHC • WB
- Reactivity:
- Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of mouse Trpm1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q7Z4N2
- Homology:
- Synthetic peptide sequence is identical to rat sequence (shows 58.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C