Trpv4 Antibody
BiCell Scientific
SKU:11034-50UL
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Protein Function
Plan your comparisons around Trpv4 and use this antibody to characterize state-dependent differences. Trpv4 is a channel or transporter associated with regulated flux across cellular membranes. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
Following Trpv4 across perturbations can help separate expression effects from compartment shifts.
Experimental Applications
Modulators that affect trafficking, phosphorylation, or ligand binding can change apparent distribution and signal intensity. Time course design can be decisive when regulation is transient or compartment dependent.
Target-centric readouts can be framed around localization, relative abundance, or complex behavior, depending on the question you are testing.
Common experimental questions that benefit from target tracking include:
- relative abundance across engineered constructs
- patterns consistent with apical or basolateral sorting
- stimulus-dependent trafficking and turnover
Workflow Perspective
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Trpv4 patterns can be compared across experiments without guesswork.
If your study spans multiple model systems, it can help to define a shared reference condition for Trpv4 so cross-sample contrasts stay meaningful.
Use the regular format option to keep Trpv4 analysis aligned with planned contrasts and endpoints.
- Targets:
- TRPV4
- Research Area:
- Cell Signaling • Neuroscience
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal region of mouse Trpv4 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9HBA0
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 88.9% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C