Phospho-Tuberin (Ser939) Antibody

About the Target

TSC4 is a target of interest in many antibody-based workflows. Phospho-Tuberin (Ser939) refers to the phosphorylated form of tuberin, the TSC2 gene product, at serine residue 939, a critical site regulated by growth factor signaling. Tuberin is a tumor suppressor protein containing functional domains for GAP activity toward Rheb, and it forms a complex with hamartin (TSC1) to inhibit mTORC1 signaling and control cell growth. Depending on the literature source, TSC4 may also be discussed as Phospho-Tuberin (Ser939) and TSC2.

Reported cellular context includes cytoplasm, lysosome, and membrane, which can matter when signal is compared across treatments or changing cell states. Following TSC4 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

TSC4 is commonly interpreted in the context of cancer, cell cycle, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm, lysosome, and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cytoplasm, lysosome, and membrane across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• cell-cycle linked differences in abundance, timing, or compartmental enrichment
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for TSC4. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in TSC4 reflect biology rather than handling. When interpreting TSC4, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep TSC4 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.