TTC21B (IFT139 or NPHP12) Antibody - FFPE Validated

About the Target

Use this antibody to investigate TTC21B when mapping complex behavior and signaling context. TTC21B is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.

In some datasets and protocols, it is referenced by the gene symbol IFT139.

Research Context

Quantitative comparisons often rely on consistent imaging parameters and clear inclusion criteria for ciliated cells. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution. Kidney disease modeling efforts may include this target when exploring podocyte stress responses and structural change.

Consider these angles when interpreting target-level changes:

• signal enrichment at basal bodies versus cytoplasm
• changes in cilia incidence or apparent length in perturbation studies
• co-occurrence with intraflagellar transport markers

Variant Considerations

Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. This matters because epitope exposure and background can vary with preparation and extraction steps. If you compare conditions, keep processing steps uniform to support clean interpretation.

When interpreting TTC21B, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

If your study spans multiple model systems, it can help to define a shared reference condition for TTC21B so cross-sample contrasts stay meaningful.

The fixed-section option supports mechanistic reading of TTC21B when interactions and context are the focus.