TTC21B (IFT139 or NPHP12) Antibody - Western Blot Validated

Scientific Overview

This antibody helps you investigate TTC21B while keeping condition-to-condition contrasts straightforward. TTC21B is a trafficking-related component that can localize to basal bodies and ciliary axonemes. Use matched handling so differences reflect biology rather than processing.

In some datasets and protocols, it is referenced by the gene symbol IFT139.

Research Relevance

Because cilia length and assembly can vary, experimental design often benefits from standardized culture timing. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood. Kidney disease modeling efforts may include this target when exploring podocyte stress responses and structural change.

When building a narrative around the target, it helps to track:

• co-occurrence with intraflagellar transport markers
• signal enrichment at basal bodies versus cytoplasm
• cell-cycle linked differences in localization
• changes in cilia incidence or apparent length in perturbation studies

Application-Specific Context

When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.

If your study spans multiple model systems, it can help to define a shared reference condition for TTC21B so cross-sample contrasts stay meaningful.

Pair this denaturing-format format with stable controls to keep TTC21B measurements coherent over time.