VAHTS Small RNA Library Prep Kit for Illumina V2

The VAHTS Small RNA Library Prep Kit for Illumina V2 from Vazyme is designed for small RNA-seq library preparation on Illumina high-throughput sequencing platforms. The kit supports the construction of sequencing-ready small RNA libraries from total RNA and purified RNA samples, including challenging low-input sample types.

Product overview

This V2 version has been optimized to improve library preparation stability and library yield at lower input amounts. During the workflow, universal adapters are ligated to the 3' and 5' ends of small RNAs, followed by reverse transcription, PCR amplification and library purification. The resulting libraries are suitable for downstream Illumina sequencing workflows.

Key features

• Small RNA-seq library preparation kit for Illumina sequencing platforms
• Optimized V2 chemistry for improved stability and library yield at low input amounts
• Suitable for animal and plant total RNA samples
• Compatible with RNA extracted from serum, plasma and exosomes
• Uses 3' and 5' adapter ligation for small RNA library construction
• Workflow includes reverse transcription, PCR amplification and library purification
• Suitable for miRNA and other small RNA sequencing applications

Applications

• Small RNA sequencing
• miRNA sequencing
• Small RNA expression profiling
• RNA biomarker research
• Exosomal RNA analysis
• Serum and plasma RNA sequencing workflows
• NGS library preparation for Illumina platforms

Compatible sample types

• Animal total RNA
• Plant total RNA
• Purified small RNA
• RNA extracted from serum
• RNA extracted from plasma
• RNA extracted from exosomes

Input material

• Animal and plant total RNA: 10 ng to 1 μg
• RNA from serum, plasma or exosomes: ≥1 ng